Stability and antibacterial properties of green synthesis silver nanoparticles using nephelium lappaceum peel extract(Article) [Kestabilan dan ciri antibakteria bagi sintesis hijau nanopartikel perak menggunakan ekstrak kulit nephelium lappaceum]

Abstract

Silver nanoparticles (AgNPs) are known for its easy production with wide range of applications. The production can involve chemical, physical, biological and photochemical methods. For production of AgNPs using biological method, the synthesis normally involves plants, bacteria or other potential reducing agents that can control the size of AgNPs. In this study, Nephelium lappaceum or also known as rambutan peel was used as a reducing agent. Nephelium lappaceum extract was added to AgNO3 (1 mM) solution and stirred for 1 hour. Three sets of synthesis were carried out to see the reproducibility and stability of the AgNPs. The solution obtained was analyzed by using Fourier transform-infrared spectroscopy (FTIR), UV-Vis spectrophotometer and scanning electron microscope (SEM). The stability of the obtained AgNPs was observed and compared with AgNPs synthesized by using the chemical method. FTIR spectra showed O-H, C-H, C=O and C=C stretching at 3361 cm-1, 2090 cm-1, 1637 cm-1 and 1367 cm-1, respectively. The wavelength of UV-Vis obtained for the 3 sets of green synthesis is at 452 nm. The appearance of the peak around 452 nm due to the surface plasmon resonance band confirmed the formation of AgNPs. SEM images showed a mixture of spherical shapes that were agglomerated with size range of about 40 to 200 nm. The antibacterial study performed on AgNPs produced from Nephelium lappaceum peel extract was found to exhibited antibacterial activity on both Gram-positive and Gram-negative bacteria.