Antioxidant Activity and Hplc Analysis of Extract from Ampelocissus Cinnamomea (Vitaceae) Tuber

Abstract

In the ongoing quest for a powerful antioxidant to neutralize free radicals, we investigate the potential antioxidant activity of the tuber of Ampelocissus cinnamomea (Vitaceae). The tuber was used to increase the endurance of the Temiar ethnicity. The objectives of this study were to determine antioxidant activity, total phenolic content (TPC), total flavonoid content (TFC), and to provide a HPLC chromatogram of the tuber extracts. The powdered tuber was extracted consecutively with petroleum ether, ethyl acetate and methanol to produce petroleum ether (EP) extract, ethyl acetate (EA) extract and methanol (ME) extract. The antioxidant activity, TPC and total TFC in the extracts were measured with 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteau and aluminium chloride, respectively. The extract profile was identified by the HPLC. The concentration of PE, EE and ME that scavenge 50% of DPPH free radicals (IC50) were ≥ 100, 12.52 ± 2.53 and 20.37 ± 3.12 μg/ml, respectively. The TPC in PE, EE and ME were 13.333 ± 3.055, 713.894 ± 28.666 and 708.439 ± 17.082 μg gallic acid equivalent/mg extract, respectively. The TFC in PE, EE and ME were 3.23 ± 1.33, 99.51 ± 3.19 and 115.53 ± 4.46 μg quercetin equivalent /mg extract, respectively. The HPLC chromatogram of the extracts revealed several major peaks that their UV-Vis spectrum resembles the class of flavonoids. In conclusion, the results were suggested to investigate in more detail an antioxidant potential in several antioxidant testing systems and isolate the active compound or compounds.