A Preliminary Study of Evaluation of the Role of Interleukin-1 beta (IL-1 beta) and Effects on Post-Treatment in Goats Experimentally Induced for Mannheimiosis

Abstract

Pro-inflammatory cytokines are necessary for an inflammatory response to infection or tissue injury. Interleukin-1β (IL-1β) is a pro-inflammatory cytokine involved in the pathophysiology of acute lung damage and respiratory distress syndrome. The study aims to evaluate the effect of treatments on the levels of IL-1β in goats induced with Mannheimiosis. Twenty male goats were divided into five groups (n = 4). All groups except group 1 (negative control) were inoculated intranasally with Mannheimia haemolytica (107 CFU/mL). Goats in group 2 served as positive control. Goats in groups 3 and 4 were treated with an antimicrobial (oxytetracycline, SID) on days 6 and 9 of post-infection and an anti-inflammatory drug (Flunixin meglumine, BID) for 5 days of post-infection, respectively. Goats in group 5 received both treatments. Blood samples were collected via jugular vein at 24 hours, days 5, 9, and 11 of post bacteria inoculation for interleukin-1β analysis. The levels of IL-1β within 24 hours and day 5 of inoculations were significantly increased (p < .05) in groups (2, 3, 4, 5) compared to the negative control. On days 9 and 11, the levels of IL-1β in groups 2 and 3 remained significantly higher (p < .05) than in other groups. These findings indicated a synergistic effect of antibiotic and anti-inflammatory drugs combination in reducing the levels of IL-1β in the blood in goats induced with Mannheimia haemolytica.