Production and application of thermostable protease 50a as liquid protein stain remover

Abstract

Proteases are important enzymes involved in many vital physiological processes and has wide potential for industrial applications. Previously, thermostable alkaline protease 50a was reported and had potential as laundry detergent additive and eco-friendly enzymatic dehairing of animal hides. Thus, exploration of this enzyme potentials' need to be done in order to prove that it plays an important role in diverse industrial application. This study aimed to investigate the potential of the thermostable alkaline protease 50a as a protein stain remover. One formulation has been developed as liquid spray protein stain remover containing the enzyme and tested its ability to remove protein stain through washing performance. Four different types of fabric which were jersey, cotton, koshibo and crepe has been stained with blood as the protein source. Blood stain on the fabrics were then removed with four different solution; using distilled water only, distilled water with enzyme, formulated liquid spray with enzyme, and formulated liquid spray without enzyme. The formulated liquid spray with enzyme exhibited better removal bloodstains action by showing faintness of the blood stained on the fabrics after it was sprayed. The stains removal evaluation on different types of fabric with formulated liquid spray protein stain remover obtained are as followed order: crepe > jersey > cotton > koshibo. This study proved that the formulated liquid spray with addition of enzyme showed the most effective stain removal on crepe fabric.